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treatment human renal proximal tubular epithelial cells  (ATCC)


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    ATCC treatment human renal proximal tubular epithelial cells
    Treatment Human Renal Proximal Tubular Epithelial Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 4550 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/treatment+human+renal+proximal+tubular+epithelial+cells/HK-2/pm41957810-85-4-15
    Average 99 stars, based on 4550 article reviews
    treatment human renal proximal tubular epithelial cells - by Bioz Stars, 2026-09
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    Cell Culture:

    Article Title: Targeting CH25H/25HC-ferroptosis axis: a novel mechanism of MSC-EVs mediated renoprotection in ischemic AKI.
    Article Snippet: .. HK-2 Cell Culture and treatment Human renal proximal tubular epithelial cells (HK-2) were purchased from American Type Culture Collection and cultured in DMEM/F12 (C11330, Gibco, USA) supplemented with 10% fetal bovine serum (10099-141C, Gibco, ThermoFisher Scentific, USA) and 1% PSA (PSA, BL141A, Biosharp, China) in a humidified atmosphere of 5% CO2 at 37 °C. ..

    Article Title: 1,25-(OH) 2 D 3 and its analogue BXL-628 inhibit high glucose-induced activation of RhoA/ROCK pathway in HK-2 cells
    Article Snippet: .. Cell culture and treatment Human renal proximal tubular epithelial cells, HK-2 (ATCC, Manassas, VA, US), were cultured in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum (Thermo Fisher Scientific, Inc., Waltham, MA, USA), streptomycin (100 μg/ml) and penicillin (100 U/ml) in a humidified atmosphere containing 5% CO 2 at 37°C. .. When cells reached 80% confluence, they were treated with high glucose (HG group; 30 mmol/l; Henyang Kaixin Chemical Reagent Company, Ltd., Hunan, China) for 0, 0.5, 1, 2, 4, 8 and 12 h to verify whether high glucose conditions activated RhoA.

    Modification:

    Article Title: 1,25-(OH) 2 D 3 and its analogue BXL-628 inhibit high glucose-induced activation of RhoA/ROCK pathway in HK-2 cells
    Article Snippet: .. Cell culture and treatment Human renal proximal tubular epithelial cells, HK-2 (ATCC, Manassas, VA, US), were cultured in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum (Thermo Fisher Scientific, Inc., Waltham, MA, USA), streptomycin (100 μg/ml) and penicillin (100 U/ml) in a humidified atmosphere containing 5% CO 2 at 37°C. .. When cells reached 80% confluence, they were treated with high glucose (HG group; 30 mmol/l; Henyang Kaixin Chemical Reagent Company, Ltd., Hunan, China) for 0, 0.5, 1, 2, 4, 8 and 12 h to verify whether high glucose conditions activated RhoA.



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    Figure 2. Polyploid tubular cells (TC) with DNA damage are profibrotic and actively produce transforming growth factor-b1 (TGF-b1) in vitro. A: barplot show- ing cell cycle phases in mouse polyploid clusters (8 and 9) divided in day 2 (t2) and 30 (t30) after unilateral ischemia reperfusion injury (uni-IRI). B: matrixplot of mouse polyploid clusters (8 and 9) divided in day 2 (t2) and 30 (t30) after uni-IRI, showing TGF-b1 and its receptors. C: Uniform Manifold Approximation and Projection (UMAP) showing human proximal tubular epithelial cell <t>(hPTC)</t> clusters. UMAP distribution of cell cycle activation (PCNA) (D), and cell cycle progression (AURKB) genes (E). F: UMAP distribution of DNA damage marker H2AFX. G–I: FACS analysis of mCherry-hPTC (cells in the G1 phase) showing the gating strategy for sorting. J: cell cycle distribution of diploid and polyploid mCherry-hPTC. A representative experiment out of 4 is shown. K: TGF-b1 gene expression in sorted polyploid mCherry-hPTC over diploid mCherry-hPTC (n ¼ 4). Statistical significance was calculated by two-sided Mann–Whitney test; numbers on graph represent exact P value. Bar plots: line¼ mean, whisker¼ outlier (coef. 1.5). n = number of experiments.
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    Figure 2. Polyploid tubular cells (TC) with DNA damage are profibrotic and actively produce transforming growth factor-b1 (TGF-b1) in vitro. A: barplot show- ing cell cycle phases in mouse polyploid clusters (8 and 9) divided in day 2 (t2) and 30 (t30) after unilateral ischemia reperfusion injury (uni-IRI). B: matrixplot of mouse polyploid clusters (8 and 9) divided in day 2 (t2) and 30 (t30) after uni-IRI, showing TGF-b1 and its receptors. C: Uniform Manifold Approximation and Projection (UMAP) showing human proximal tubular epithelial cell <t>(hPTC)</t> clusters. UMAP distribution of cell cycle activation (PCNA) (D), and cell cycle progression (AURKB) genes (E). F: UMAP distribution of DNA damage marker H2AFX. G–I: FACS analysis of mCherry-hPTC (cells in the G1 phase) showing the gating strategy for sorting. J: cell cycle distribution of diploid and polyploid mCherry-hPTC. A representative experiment out of 4 is shown. K: TGF-b1 gene expression in sorted polyploid mCherry-hPTC over diploid mCherry-hPTC (n ¼ 4). Statistical significance was calculated by two-sided Mann–Whitney test; numbers on graph represent exact P value. Bar plots: line¼ mean, whisker¼ outlier (coef. 1.5). n = number of experiments.
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    Figure 2. Polyploid tubular cells (TC) with DNA damage are profibrotic and actively produce transforming growth factor-b1 (TGF-b1) in vitro. A: barplot show- ing cell cycle phases in mouse polyploid clusters (8 and 9) divided in day 2 (t2) and 30 (t30) after unilateral ischemia reperfusion injury (uni-IRI). B: matrixplot of mouse polyploid clusters (8 and 9) divided in day 2 (t2) and 30 (t30) after uni-IRI, showing TGF-b1 and its receptors. C: Uniform Manifold Approximation and Projection (UMAP) showing human proximal tubular epithelial cell <t>(hPTC)</t> clusters. UMAP distribution of cell cycle activation (PCNA) (D), and cell cycle progression (AURKB) genes (E). F: UMAP distribution of DNA damage marker H2AFX. G–I: FACS analysis of mCherry-hPTC (cells in the G1 phase) showing the gating strategy for sorting. J: cell cycle distribution of diploid and polyploid mCherry-hPTC. A representative experiment out of 4 is shown. K: TGF-b1 gene expression in sorted polyploid mCherry-hPTC over diploid mCherry-hPTC (n ¼ 4). Statistical significance was calculated by two-sided Mann–Whitney test; numbers on graph represent exact P value. Bar plots: line¼ mean, whisker¼ outlier (coef. 1.5). n = number of experiments.
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    Figure 2. Polyploid tubular cells (TC) with DNA damage are profibrotic and actively produce transforming growth factor-b1 (TGF-b1) in vitro. A: barplot show- ing cell cycle phases in mouse polyploid clusters (8 and 9) divided in day 2 (t2) and 30 (t30) after unilateral ischemia reperfusion injury (uni-IRI). B: matrixplot of mouse polyploid clusters (8 and 9) divided in day 2 (t2) and 30 (t30) after uni-IRI, showing TGF-b1 and its receptors. C: Uniform Manifold Approximation and Projection (UMAP) showing human proximal tubular epithelial cell <t>(hPTC)</t> clusters. UMAP distribution of cell cycle activation (PCNA) (D), and cell cycle progression (AURKB) genes (E). F: UMAP distribution of DNA damage marker H2AFX. G–I: FACS analysis of mCherry-hPTC (cells in the G1 phase) showing the gating strategy for sorting. J: cell cycle distribution of diploid and polyploid mCherry-hPTC. A representative experiment out of 4 is shown. K: TGF-b1 gene expression in sorted polyploid mCherry-hPTC over diploid mCherry-hPTC (n ¼ 4). Statistical significance was calculated by two-sided Mann–Whitney test; numbers on graph represent exact P value. Bar plots: line¼ mean, whisker¼ outlier (coef. 1.5). n = number of experiments.
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    treatments human renal proximal tubular epithelial cells  (ATCC)
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    Figure 2. Polyploid tubular cells (TC) with DNA damage are profibrotic and actively produce transforming growth factor-b1 (TGF-b1) in vitro. A: barplot show- ing cell cycle phases in mouse polyploid clusters (8 and 9) divided in day 2 (t2) and 30 (t30) after unilateral ischemia reperfusion injury (uni-IRI). B: matrixplot of mouse polyploid clusters (8 and 9) divided in day 2 (t2) and 30 (t30) after uni-IRI, showing TGF-b1 and its receptors. C: Uniform Manifold Approximation and Projection (UMAP) showing human proximal tubular epithelial cell <t>(hPTC)</t> clusters. UMAP distribution of cell cycle activation (PCNA) (D), and cell cycle progression (AURKB) genes (E). F: UMAP distribution of DNA damage marker H2AFX. G–I: FACS analysis of mCherry-hPTC (cells in the G1 phase) showing the gating strategy for sorting. J: cell cycle distribution of diploid and polyploid mCherry-hPTC. A representative experiment out of 4 is shown. K: TGF-b1 gene expression in sorted polyploid mCherry-hPTC over diploid mCherry-hPTC (n ¼ 4). Statistical significance was calculated by two-sided Mann–Whitney test; numbers on graph represent exact P value. Bar plots: line¼ mean, whisker¼ outlier (coef. 1.5). n = number of experiments.
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    Figure 2. Polyploid tubular cells (TC) with DNA damage are profibrotic and actively produce transforming growth factor-b1 (TGF-b1) in vitro. A: barplot show- ing cell cycle phases in mouse polyploid clusters (8 and 9) divided in day 2 (t2) and 30 (t30) after unilateral ischemia reperfusion injury (uni-IRI). B: matrixplot of mouse polyploid clusters (8 and 9) divided in day 2 (t2) and 30 (t30) after uni-IRI, showing TGF-b1 and its receptors. C: Uniform Manifold Approximation and Projection (UMAP) showing human proximal tubular epithelial cell (hPTC) clusters. UMAP distribution of cell cycle activation (PCNA) (D), and cell cycle progression (AURKB) genes (E). F: UMAP distribution of DNA damage marker H2AFX. G–I: FACS analysis of mCherry-hPTC (cells in the G1 phase) showing the gating strategy for sorting. J: cell cycle distribution of diploid and polyploid mCherry-hPTC. A representative experiment out of 4 is shown. K: TGF-b1 gene expression in sorted polyploid mCherry-hPTC over diploid mCherry-hPTC (n ¼ 4). Statistical significance was calculated by two-sided Mann–Whitney test; numbers on graph represent exact P value. Bar plots: line¼ mean, whisker¼ outlier (coef. 1.5). n = number of experiments.

    Journal: American journal of physiology. Cell physiology

    Article Title: Polyploid tubular cells initiate a TGF-β1 controlled loop that sustains polyploidization and fibrosis after acute kidney injury.

    doi: 10.1152/ajpcell.00081.2023

    Figure Lengend Snippet: Figure 2. Polyploid tubular cells (TC) with DNA damage are profibrotic and actively produce transforming growth factor-b1 (TGF-b1) in vitro. A: barplot show- ing cell cycle phases in mouse polyploid clusters (8 and 9) divided in day 2 (t2) and 30 (t30) after unilateral ischemia reperfusion injury (uni-IRI). B: matrixplot of mouse polyploid clusters (8 and 9) divided in day 2 (t2) and 30 (t30) after uni-IRI, showing TGF-b1 and its receptors. C: Uniform Manifold Approximation and Projection (UMAP) showing human proximal tubular epithelial cell (hPTC) clusters. UMAP distribution of cell cycle activation (PCNA) (D), and cell cycle progression (AURKB) genes (E). F: UMAP distribution of DNA damage marker H2AFX. G–I: FACS analysis of mCherry-hPTC (cells in the G1 phase) showing the gating strategy for sorting. J: cell cycle distribution of diploid and polyploid mCherry-hPTC. A representative experiment out of 4 is shown. K: TGF-b1 gene expression in sorted polyploid mCherry-hPTC over diploid mCherry-hPTC (n ¼ 4). Statistical significance was calculated by two-sided Mann–Whitney test; numbers on graph represent exact P value. Bar plots: line¼ mean, whisker¼ outlier (coef. 1.5). n = number of experiments.

    Article Snippet: Data were analyzed by FlowLogic software (FlowLogic 7.2.1, Inivai Technology). hPTC Culture, Virus Transduction, FluorescenceActivated Cell Sorting, TGF-b1, Verteporfin, and Fresolimumab Treatments Human proximal tubular cells (hPTC) (ATCC-PCS-400010) were maintained in renal epithelial growth medium (REGM) (Lonza, CC-3190). hPTC were seeded at a density of 105 cells/6-well.

    Techniques: In Vitro, Activation Assay, Marker, Gene Expression, MANN-WHITNEY

    Figure 5. Tubular cells (TC) interact with monocytes and fibroblasts activating proinflammatory and profibrotic pathways. A: schematic representation of experimental plan for coculture with monocytes. Real-time PCR analysis of hypoxia-inducible factor (HIF)1a (B), MAF(C), IL-6 (D), and transforming growth factor (TGF)-b1 (E) in monocytes following coculture with nonstimulated human proximal tubular epithelial cells (hPTC) and after TGF-b1 and H2O2 treat- ment (n ¼ 4). F: schematic representation of experimental plan for coculture with fibroblasts. G and H: real-time PCR analysis of p21 and CCL2 in fibro- blasts following coculture with nonstimulated hPTC and after TGF-b1 and H2O2 treatment (n ¼ 4). Statistical significance was calculated by two-sided Mann–Whitney test; numbers on graphs represent exact P values. Bar plots: line¼ mean, whisker ¼ outlier (coef. 1.5). Empty: transwell with monocytes or fibroblasts without hPTC; t0: monocytes after purification. n = number of experiments; MAF, musculoaponeurotic fibrosarcoma.

    Journal: American journal of physiology. Cell physiology

    Article Title: Polyploid tubular cells initiate a TGF-β1 controlled loop that sustains polyploidization and fibrosis after acute kidney injury.

    doi: 10.1152/ajpcell.00081.2023

    Figure Lengend Snippet: Figure 5. Tubular cells (TC) interact with monocytes and fibroblasts activating proinflammatory and profibrotic pathways. A: schematic representation of experimental plan for coculture with monocytes. Real-time PCR analysis of hypoxia-inducible factor (HIF)1a (B), MAF(C), IL-6 (D), and transforming growth factor (TGF)-b1 (E) in monocytes following coculture with nonstimulated human proximal tubular epithelial cells (hPTC) and after TGF-b1 and H2O2 treat- ment (n ¼ 4). F: schematic representation of experimental plan for coculture with fibroblasts. G and H: real-time PCR analysis of p21 and CCL2 in fibro- blasts following coculture with nonstimulated hPTC and after TGF-b1 and H2O2 treatment (n ¼ 4). Statistical significance was calculated by two-sided Mann–Whitney test; numbers on graphs represent exact P values. Bar plots: line¼ mean, whisker ¼ outlier (coef. 1.5). Empty: transwell with monocytes or fibroblasts without hPTC; t0: monocytes after purification. n = number of experiments; MAF, musculoaponeurotic fibrosarcoma.

    Article Snippet: Data were analyzed by FlowLogic software (FlowLogic 7.2.1, Inivai Technology). hPTC Culture, Virus Transduction, FluorescenceActivated Cell Sorting, TGF-b1, Verteporfin, and Fresolimumab Treatments Human proximal tubular cells (hPTC) (ATCC-PCS-400010) were maintained in renal epithelial growth medium (REGM) (Lonza, CC-3190). hPTC were seeded at a density of 105 cells/6-well.

    Techniques: Real-time Polymerase Chain Reaction, MANN-WHITNEY, Whisker Assay